Table 5 APOE and TREM2 Models
From: Practical considerations for choosing a mouse model of Alzheimer’s disease
Model | Allele | Targeting approach | Availability | Reference |
|---|---|---|---|---|
ApoE Lines | ||||
GFAP-ApoE3 Line 37, GFAP-ApoE4, Line 1 | huAPOE3 or E4 cDNA | Transgenic, huGFAP promoter | JAX 004633, 004631 | [196] |
ApoE2, E3, E4 KI | huAPOE2, E3, or E4 | Targeted insertion of APOE cDNAs | NA | [197] |
ApoE KO | Apoe deletion (exon 3 replacement) | Targeted neo insertion replacing part of Apoe exon 3 | JAX 002052; 014556 | |
APOE*3, E*4 KI | huAPOE3 or E4 | Targeted replacement of Apoe exons 2–4 | JAX 027894 (E4) JAX 029018 (E3) | NA |
APOE2, E3, E4 Targeted replacement | huAPOE2, E3, or E4 | Targeted replacement of Apoe exons 2–4 | Taconic | |
TREM2 Lines | ||||
TREM2 KO | Trem2 deletion (exon 2–3 deletion) | Targeted lacZ/neo replacement of TREM2 exons 2–4 | UCD/KOMP VG10093 | [206] |
TREM2−/− | Trem2 deletion (exon 2–3 deletion) | Targeted deletion TREM2 exons 3–4 | Marco Colonna | [205] |
TREM2 KO | Trem2 deletion (Q17X) | CRISPR/Cas9 targeted deletion | JAX 027197 | NA |
TREM2 R47H KI | endogenous mouse Trem2 (R47H) | CRISPR/Cas9 targeted mutation | JAX 027918 | NA |
TREM2 Y38C KI | endogenous mouse Trem2 (Y38C) | CRISPR/Cas9 targeted mutation | JAX 029725 | NA |
TREM2 p.T66 M | endogenous mouse Trem2 (T66 M) | CRISPR/Cas9 targeted mutation | Christian Haass | [208] |
TREM2 flox | Trem2 tm1c(EUCOMM)Wtsi loxP-flanked mouse Trem2 exons 2–3 | Targeted insertion | JAX 029853 | NA |