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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Glucose transporter 1 critically controls microglial activation through facilitating glycolysis

Fig. 4

Mitochondrial respiration in different types of microglial cells. BV2 and B6M7 cells were untreated (naïve), or treated with LPS + IFNγor IL-4 for 24 h. The cells were then subjected to Mito Stress Test. The OCR was measured sequentially under basal conditions, following inhibition of ATP synthase (with oligomycin), uncoupling the electron transporter chain (ETC) with FCCP, and blocking complex I and III with rotenone and antimycin A. a, e Representative OCR profiles of BV2 (a) and B6M7 (e) naïve, LPS + IFNγ- or IL-4-treated cells. b, f Changes in basal OCR in LPS + IFNγ treated and IL-4 treated cells compared to naïve microglia. c, g Changes in ATP production in LPS + IFNγ- and IL-4-treated microglial cells compared to naïve microglia. d, h Changes in maximal respiration in LPS + IFNγ- and IL-4-treated microglial cells compared to naïve microglia. Mean ± SEM, n = 3 repeated experiments, **P < 0.01, ***P < 0.001

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