Fig. 7
From: Glucose transporter 1 critically controls microglial activation through facilitating glycolysis
The effect of STF31 on microglial mitochondrial respiration. B6M7 cells were incubated with 5 μM STF31, and then stimulated with LPS + IFNγ or IL-4. The cells were then subjected to Mito Stress assay (a-f) or MitoSOX Red assay (g, h). a-c Representative OCR profiles in naïve (a), M(LPS + IFNγ) (b), and M(IL-4) (c) microglia with or without 5 μM STF31. d The effect of STF31 on microglial basal respiration of naïve, M(LPS + IFNγ), and M(IL-4) microglial cells. e The effect of STF31 on ATP production of naive, M(LPS + IFNγ), and M(IL-4) microglial cells. f The effect of STF31 on spare respiratory capacity of naive, M(LPS + IFNγ), and M(IL-4) microglial cells. g, h The effect of STF31 on the production of mitochondrial superoxidate in naive, LPS + IFNγ, IL-4 treated B6M7 (g) and primary microglia (PMG, h). Mean ± SEM, n = 3 repeated experiments, *, P < 0.05; **P < 0.01; ***P < 0.001