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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Glucose transporter 1 critically controls microglial activation through facilitating glycolysis

Fig. 8

The effect of STF31 on microglial phagocytosis, cytokine expression/secretion and intracellular reactive oxygen spececis production. a B6M7 cells and primary microglia (PMG) were incubated with 5 μM STF31 for 24 h. Phagocytosis was performed using the pHrodo Green S. aureus BioParticles. Inserted images showing green fluorescence from phagocytosed S. aureus bioparticles in different groups of cells. Mean ± SEM, n = 3 (repeated experiments), *P < 0.05; **P < 0.01; ***P < 0.001. One-way ANOVA with Tukey’s Multiple Comparison Test. b-d B6M7 cells were incubated with 5 μM STF31 for 24 h followed by LPS + IFNγ or IL-4 stimulation. The mRNA expression of TNFα, IL-β, IL-6, iNOS, CCL2 and arginase-1 was measured by real-time RT-PCR (b). c The production of TNFα, CCL2, RANTES, and IL-6 was measured by CBA, and NO production was determined by Griess Assay. d The effect of STF31 on intracellular reactive oxygen species (ROS) production in naive, LPS + IFNγ, IL-4 treated B6M7 and primary microglia (PMG) meased by CellRox Green assay. Mean ± SEM, n = 3 repeated experiments, **P < 0.01; ***P < 0.001; ****P < 0.0001

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