Table 1 Primers used for qRT-PCR
From: Glucose transporter 1 critically controls microglial activation through facilitating glycolysis
Targets | Annealing temperature (°C) | Sequences (5′ - 3′) F: forward R: reverse |
|---|---|---|
β-actin | 58 | F CCTTCCTTCTTGGGTATG |
R TGTAAAACGCAGCTCAGTAA | ||
GLUT1 | 60 | F CCCCGTCCTGCTGCTATTG |
R GCACCGTGAAGATGATGAAGAC | ||
GLUT3 | 60 | F TGTCCAGGAACCGATCTATGC |
R CCACCAGGAACAGAGAAACTAC | ||
GLUT4 | 60 | F CCAGTATGTTGCGGATGCTAT |
R TTTTAGGAAGGTGAAGATGAAGAAG | ||
GLUT5 | 60 | F ACCTCAGCGCAGGCGTGAAA |
R AGCAGGCTATGAGGCAGGTGGA | ||
GLUT6 | 60 | F CGTCCAGTTTGTGCCAAGG |
R CAGCAGGGGTATCAGGGTAAG | ||
GLUT8 | 60 | F GCTCACCGCTGCCTTCTG |
R CGAAATGGGCTGTGACTTGTT | ||
GLUT9 | 60 | F ACTATGTGGACTCAATGCGATCT |
R TCAATGACCAAGCCAGAGAAGA | ||
GLUT10 | 60 | F CTCTGTGGCGTTCATCAAGTG |
R ACGAGGAAGGCGGAGACT | ||
GLUT12 | 60 | F TGGCAGCAAAACCTTCCTCT |
R AGGGACTGGTTAAGAAGACTATGG | ||
GLUT13 | 60 | F CTGGAATAAACTGGATTTTCAACG |
R TCAGGGAGGCAGCCATAGA | ||
Arginase 1 | 61 | F TTATCGGAGCGCCTTTCTCAA |
R TGGTCTCTCACGTCATACTCTGT | ||
CCL2 | 58 | F AGGTCCCTGTCATGCTTCTG |
R TCTGGACCCATTCCTTCTT | ||
IL-1β | 61 | F TCCTTGTGCAAGTGTCTGAAGC |
R ATGAGTGATACTGCCTGCCTGA | ||
iNOS | 60 | F GGCAAACCCAAGGTCTACGTT |
R TCGCTCAAGTTCAGCTTGGT | ||
TNF-α | 58 | F GCCTCTTCTCATTCCTGCTT |
R CTCCTCCACTTGGTGGTTTG | ||
IL-12P40 | 60 | F GACATCATCAAACCAGACCCGCC |
R GCCTTTGCATTGGACTTCGGT |